Thrombodynamics Analyser System
Real-time visualization of clot growth
Thrombodynamics Analyser System is intended to provide qualitative and quantitative evaluation of the coagulation state of a blood plasma sample.
- Reconstruction of blood vessel wall damage in vitro
- Physiological activation of coagulation
- Real-time observation of fibrin clot propagation
- Considers not only biochemical reactions of the coagulation cascade but also spatial aspects of the clot formation process
- Reveals both hypo- and hypercoagulation states
- Highest sensitivity to all clasic anticoagulants (heparins, warfarin)
Fibrin clot formation
The main idea of Thrombodynamics assay is based on spatial separation of activation and propagation phases of coagulation.
The coagulation is activated by a special surface with immobilized tissue factor (TF), which reconstructs damage vessel wall. As soon as blood plasma sample comes into contact with TF, the coagulation process initiates and fibrin clot starts growing in a thin layer of non-stirred plasma, placed into a plastic cuvette.
Clot formation starts on the surface of the activator and propagates into the bulk of plasma. Images of clot formation are registered via CCD camera using a time lapse microscopy mode in scattered light.
Software calculates the parameters of Thrombodynamics.
Thrombodynamics Analyser allows visualization of the fibrinolysis process in presence of plasminogen activators (tPA or uPA).
The initiation phase of clot formation occurs similarly to the basic Thrombodynamics setup. Further coagulation propagation can be observed simultaneously with the lysis process.
An example of time lapse images of the fibrin clot growth and lysis with ~60 units / mL of uPA is shown on the figure.
In addition to registering fibrin clot growth from the immobilized coagulation activator, Thrombodynamics-4D simultaneously allows registering spatiotemporal dynamics of thrombin, the main enzyme of the coagulation cascade, in parallel with spatial fibrin clot growth registration from the tissue factor bearing surface.
Registration of thrombin formation is based on fluorescent microscopy principle, using an AMC-based fluorogenic substrate,
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Comparison of the Thrombodynamics Analyser Systems: