The First Recombinant Cascade LAL Reagent
NODIA and it’s partner Associates of Cape Cod, Inc. (ACC), are excited to let you know that PyroSmart NextGen™, The Future of Sustainable LAL Recombinant Cascade Reagent Has Arrived!
- Same Preparation steps
- Same Instrument
- Same Cascade
- No Animal Content – Horseshoe Crab Blood Free.
- Shorter reaction time (2500s for 0.005EU/ml sensitivity)
Keep your Method - Make an Impact
PyroSmart NextGen™ recombinant Cascade Reagent (rCR) marks the introduction of a new sustainable recombinant LAL reagent technology for Bacterial Endotoxin Testing (BET). Utilizing the same LAL cascade as traditional LAL reagents, while eliminating the potential for 1,3-β-D-glucans cross reactivity, PyroSmart NextGen™ delivers all of the quality and consistency of results you have come to expect from ACC LAL reagents.
PyroSmart NextGen™ can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.
The sensitivity for recombinant chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of PyroSmart NextGen™ is 0.005 EU/mL when run in an incubating microplate reader (or 0.001 EU/mL when run in Pyros Kinetix® Flex tube reader).
Traditional vs Next generation
|Conventional LAL Reagent||PyroSmart NextGen™ (rCR)||Competitor (rFC) Reagent|
|Year Technology Introduced||1977||2021||2005|
|Kinetic Assay||Kinetic||Kinetic||Endpoint only|
|Assay Setup||Single step reconstitution||Single step reconstitution||Requires three reagents in a 1:4:5 ratio and a 10 min pre-incubation step|
|Same Standard Plate Reader||Incubating plate or tube reader at 405nm||Incubating plate or tube reader at 405nm||Fluorescent reader required|
Limulus Amebocyte Lysate (LAL)
|LAL||rCR is recombinant LAL||Based on Carcinoscorpius or Tachypleus
Amebocyte Lysate (CAL/TAL)
|Multi-step Cascade Pathway||Yes||Yes||No|
|Endotoxin Specific||No||Endotoxin Specific||Endotoxin Specific|
|Sustainable Reagent (animal free)||No||Horseshoe Crab Blood Free||Horseshoe Crab Blood Free|
Sample to lysate Ratio
PyroSmart NextGen™ is used at with an economical volume of 50 µL of reagent per well yielding 50 tests/vial:
- Microplate reader: 1:1 ratio using 50 µL of test sample : 50 µL of reagent
- Pyros Kinetix® Flex tube reader: 4:1 using 200 µL of test sample : 50 µL of reagent
The PyroSmart NextGen™ reaction mixture is incubated at 37±1°C and read in a microplate reader equipped with a 405–410 nm filter. The time of incubation is dependent on the lowest standard concentration in the standard curve, with 0.005 EU/mL achievable in 2500 seconds in a microplate reader. Software is used to construct the standard curve and calculate the endotoxin concentrations.
PyroSmart NextGen™ is provided as co-lyophilized with the chromogenic substrate and as such it is ready-to-use following a simple reconstitution (using 2.8mL of the supplied reconstitution buffer).
PyroSmart NextGen™ is a lyophilized product with an excellent shelf life of 3 years since the date of manufacture. 2.8 mL/vial (approx. 50 tests/vial) (#PNG050-2).
- 2 x PyroSmart NextGen™ Reagent
- 2 x Reconstitution Buffer
This is sufficient for a total of 110 wells (55 wells per vial).