PyroSmart NextGen™

The First Recombinant Cascade LAL Reagent

NODIA and it’s partner Associates of Cape Cod, Inc. (ACC), are excited to let you know that PyroSmart NextGen™, The Future of Sustainable LAL Recombinant Cascade Reagent Has Arrived!

  • Same Preparation steps
  • Same Instrument
  • Same Cascade

  • No Animal Content – Horseshoe Crab Blood Free. 
  • Shorter reaction time (2500s for 0.005EU/ml sensitivity) 

Keep your Method - Make an Impact

General

Product Description

PyroSmart NextGen™ recombinant Cascade Reagent (rCR) marks the introduction of a new sustainable recombinant LAL reagent technology for Bacterial Endotoxin Testing (BET). Utilizing the same LAL cascade as traditional LAL reagents, while eliminating the potential for 1,3-β-D-glucans cross reactivity, PyroSmart NextGen™ delivers all of the quality and consistency of results you have come to expect from ACC LAL reagents.

PyroSmart NextGen™ can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.

PyroSmart NextGen cascade
PyroSmart NextGen pijl

Product Sensitivity

The sensitivity for recombinant chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of PyroSmart NextGen™ is 0.005 EU/mL when run in an incubating microplate reader (or 0.001 EU/mL when run in Pyros Kinetix® Flex tube reader).

Traditional vs Next generation

 Conventional LAL ReagentPyroSmart NextGen™ (rCR)Competitor (rFC) Reagent
Year Technology Introduced197720212005
Kinetic AssayKineticKineticEndpoint only
Assay SetupSingle step reconstitutionSingle step reconstitutionRequires three reagents in a 1:4:5 ratio and a 10 min pre-incubation step
Same Standard Plate ReaderIncubating plate or tube reader at 405nmIncubating plate or tube reader at 405nm Fluorescent reader required
Derived From:
Limulus Amebocyte Lysate (LAL)
LALrCR is recombinant LALBased on Carcinoscorpius or Tachypleus
Amebocyte Lysate (CAL/TAL)
Multi-step Cascade PathwayYesYesNo
Endotoxin SpecificNoEndotoxin SpecificEndotoxin Specific
Sustainable Reagent (animal free)NoHorseshoe Crab Blood FreeHorseshoe Crab Blood Free

Extra Information

Sample to lysate Ratio

PyroSmart NextGen™ is used at with an economical volume of 50 µL of reagent per well yielding 50 tests/vial:

  • Microplate reader: 1:1 ratio using 50 µL of test sample : 50 µL of reagent
  • Pyros Kinetix® Flex tube reader: 4:1 using 200 µL of test sample : 50 µL of reagent

Test Performance

The PyroSmart NextGen™ reaction mixture is incubated at 37±1°C and read in a microplate reader equipped with a 405–410 nm filter. The time of incubation is dependent on the lowest standard concentration in the standard curve, with 0.005 EU/mL achievable in 2500 seconds in a microplate reader. Software is used to construct the standard curve and calculate the endotoxin concentrations.

Product Reconstitution

PyroSmart NextGen™ is provided as co-lyophilized with the chromogenic substrate and as such it is ready-to-use following a simple reconstitution (using 2.8mL of the supplied reconstitution buffer).

Product Packaging

PyroSmart NextGen™ is a lyophilized product with an excellent shelf life of 3 years since the date of manufacture. 2.8 mL/vial (approx. 50 tests/vial)  (#PNG050-2). 

  • 2 x PyroSmart NextGen™ Reagent
  • 2 x Reconstitution Buffer
 

 This is sufficient for a total of 110 wells (55 wells per vial).

Frequently Asked Questions

1. Will PSNG perform the same as naturally sourced reagents?

Yes, PSNG is a kinetic chromogenic reagent. It contains recombinant Factor C, Factor B and pro-clotting enzyme, thus it continues to rely on the cascade mechanism in order to amplify the signal. It is co-lyophilized with the same chromogenic substrate as other chromogenic reagents. Therefore it is homogeneous with Pyrochrome® and Chromo-LAL. This presents a huge advantage to the end user: they keep the method, only switch out the reagent.

2. What’s the difference between Recombinant Factor C (rFC) and Recombinant Cascade Reagent (rCR)?

Recombinant Factor C reagent contains only recombinant Factor C. This enzyme acts as a biosensor for endotoxin (specifically binds to it). But since the cascade amplifying the signal is missing (no Factor B and pre-clotting enzyme), Factor C reagents are used in combination with fluorescence read out in order to achieve sensitivity levels comparable to the cascade reaction. This means that a fluorescent plate reader has to be used (along with a specific microplate). So this means different method, different readers, different SOPs for rFC reagents. Recombinant cascade reagent (e.g. recombinant TAL (= PyroSmart) or recombinant LAL (= PyroSmart NextGen™) are homogenous to current LAL reagents (Pyrochrome® or Chromo-LAL) in that they contain the full cascade (3-enzyme cascade).

3. Does PSNG use any raw material from the horseshoe crab?

No. PSNG is completely animal free.

4. Will new instrumentation be required to use PSNG?

No. Due to the cascade based feature, you can utilize the same instrument and preparation procedures.

5. How are recombinant reagents produced?

It is a biopharmaceutical production based on the use of expression system as cell line clones, that contain (thanks to genetic engeering) DNA coding Factor C, Factor B and pro-clotting enzymes from Limulus Polyphemus. The cell lines express the final glycoproteins Factor C, Factor B and pro-clotting enzyme which are then collected, purified and formulated into PyroSmart NextGen™.

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