Sandwich ELISA assay for the measurement of human PF4 (platelet factor 4), in platelet poor human plasma, or any biological sample where Platelet Factor 4 must be measured. Assay designed with affinity purified rabbit polyclonal antibodies for coating the MicroELISA plate (immunocapture) as well as preparing the Horse Radish Peroxidase (HRP) immunoconjugate marker. This assay measures native or recombinant Platelet Factor 4. It is insensitive to the presence of heparin in the tested sample, and Platelet Factor 4 is measured homogeneously whether its presentation is. The assay calibrator is standardized with the NIBSC International Standard.
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