Skip to content

Lysis Timer

A new sensitive tool to explore Fibrinolysis

For the evaluation of the Global Fibrinolytic Capacity (GFC)

  • Standardized Test Protocol
  • Uses only 100µl of Citrated Plasma
  • Easy two-step semi-automated assay
  • Sensitive to both hyper- and hypo-fibrinolysis
  • High, Normal and Low Controls available
  • One test provides a full fibrinolytic profile in 1 hour
  • Strong correlation with underlying fibrinolysis parameters
  • Sensitive to tPA related fibrinolysis deviations



Fibrinolysis plays a crucial roll in maintaining an equilibrium in blood circulation. An unbalance in the fibrinolysis system can cause a wide variety of clinical problems such as thrombotic risk, extensive bleeding and inflammation.

Most of the proteins involved in fibrinolysis can be measured using immunoassays or by using chromogenic substrates. Other functional assays have been reported as well.

Until now, it has always been time-consuming, difficult and incomplete to explore fibrinolysis. Although many attempts have been made developing a good global fibrinolysis assay, the delayed onset and slow reaction mechanism have complicated the development of such an assay.

Hyphen BioMed has introduced recently a new approach of global fibrinolysis capacity (GFC), based on an existing method developed and reported by Zouaoui et al. The diagnostic reagent was optimized and a dedicated instrument and software were developed by Hyphen BioMed.

The GFC/LT method has the advantage to provide results in less than 1 hour and provides better performances than viscoelastic tests.

Today, the Lysis Timer (LT) instrument, paired with the GFC reagents, can fill this gap by providing fast and reliable results.

Device and Software

Photometer permits the real-time follow-up of light
transmittance (940nm) in independent tubes thermostated at 37°C.

The intuitive software interface allows real-time monitoring of the derivation curve: the Lysis Time.


Stability of GFC-test and GFC control plasmas after reconstitution give a great work flexibility:

  • 24 hours at 2-8°C
  • 8 hours at 18-25°C
  • 1 month at -20°C for GFC-test
  • 2 months at -20°C for GFC control.
LT Reagens


Reaction principle

From the original method reported by Zouaoui et Al., Hyphen BioMed has designed an assay for evaluating the global fibrinolytic capacity (GFC), including the diagnostic reagent, a dedicated instrument and a software.


Fibrinolysis Related Products

ReferenceProduct namePackagingCategory
LT-8Lysis TimerSystemInstrument
CK093KGFC-test3 x 20 testsGFC-assay
SC104KGFC Control Plasma (3 levels) 2 x 10 tests
221511BIOPHEN Plasminogen LRT3 x 3mlChromogenic assay
220502BIOPHEN a2-Antiplasmin LRT3 x 3mlChromogenic assay
521296ZYMUPHEN Tissue Plasminogen Activator (tPA) Activity (on-demand)95 testsBio-Immuno Assay
RK008AZYMUTEST™ (Total) TAFI Ag96 testsELISA
RK037AZYMUTEST™ (Activatable) TAFI96 testsELISA
RK019AZYMUTEST™ PAI-1 activity96 testsELISA
SC011KPlasma PAI-1 control2x (3x1ml)Controls
SC017KPlasma UTA control (uPA/tPA/Annexin V)2x (3x1ml)Controls
5D-42154FHuman Plasminogen Deficient Plasma, Frozen1mlDeficient Plasma
5D-42154LHuman Plasminogen Deficient Plasma, Lyophilized1mlDeficient Plasma
5D-42150FHuman PAI-1 Deficient Plasma, Frozen1mlDeficient Plasma
5D-42250FHuman TPA Deficient Plasma, Frozen1mlDeficient Plasma
5D-42255FHuman TPA and PAI-1 Deficient Plasma, Frozen1mlDeficient Plasma
5D-42233FHuman TAFI Deficient Plasma, Frozen1mlDeficient Plasma
5D-42233LHuman TAFI Deficient Plasma, Lyophilized1mlDeficient Plasma

Interesting literature

Amiral, J., Laroche, M., & Seghatchian, J. (2018). A new assay for global fibrinolysis capacity (GFC): Investigating a critical system regulating hemostasis and thrombosis and other extravascular functions. Transfusion and Apheresis Science, 57(1), 118–126.

Bareille, M., Hardy, M., Chatelain, B., Lecompte, T., & Mullier, F. (2022). Laboratory evaluation of a new integrative assay to phenotype plasma fibrinolytic system. Thrombosis Journal, 20(1), 73.

Bareille M, Lecompte T, Mullier F. Assessment of the individual determinants of two fibrinolysis assays, GFC and ECLT in platelet-depleted plasma and derived euglobulin fraction respectively. Thromb Res. 2023 Oct;230:33-36. Epub 2023 Aug 13. PMID: 37633059. doi: 10.1016/j.thromres.2023.08.008. 

Lampridou M, Sokou R, Tsantes AG, Theodoraki M, Konstantinidi A, Ioakeimidis G, Bonovas S, Politou M, Valsami S, Iliodromiti Z, Boutsikou T, Iacovidou N, Nikolopoulos G, Tsantes AE. ROTEM diagnostic capacity for measuring fibrinolysis in neonatal sepsis. Thromb Res. 2020 Aug;192:103-108. Epub 2020 May 20. PMID: 32464452. doi: 10.1016/j.thromres.2020.05.028. 

Raynaud, F., Rousseau, A., Monteyne, D., Perez-Morga, D., Zouaoui Boudjeltia, K., & Chopard, B. (2021). Investigating the two regimes of fibrin clot lysis: An experimental and computational approach. Biophysical Journal, 120(18), 4091–4106.

Rossetto A, Torres T, Platton S, Vulliamy P, Curry N, Davenport R. A new global fibrinolysis capacity assay for the sensitive detection of hyperfibrinolysis and hypofibrinogenemia in trauma patients. J Thromb Haemost. 2023 Oct;21(10):2759-2770. Epub 2023 May 17. PMID: 37207863. doi: 10.1016/j.jtha.2023.05.005.

Roullet, S., Labrouche, S., Mouton, C., Quinart, A., Nouette-Gaulain, K., Laurent, C., & Freyburger, G. (2019). Lysis Timer: A new sensitive tool to diagnose hyperfibrinolysis in liver transplantation. Journal of Clinical Pathology72(1), 58–65.

Roullet S, Labrouche S, Freyburger G. Fibrinolysis during liver transplantation: analysis by the Thrombodynamics method. J Clin Pathol. 2019 Sep;72(9):636-638. Epub 2019 Apr 11. PMID: 30975704. doi: 10.1136/jclinpath-2018-205560.

Roullet S, Weinmann L, Labrouche S, Gisbert-Mora C, Biais M, Revel P, Freyburger G. Fibrinolysis in trauma patients: wide variability demonstrated by the Lysis Timer. Scand J Clin Lab Invest. 2019 Feb-Apr;79(1-2):136-142. Epub 2019 Mar 12. PMID: 30861350. doi: 10.1080/00365513.2019.1584829.

Tsuchida T, Hayakawa M, Kumano O. Comparison of results obtained using clot-fibrinolysis waveform analysis and global fibrinolysis capacity assay with rotational thromboelastography. Sci Rep. 2024 Mar 31;14(1):7602. PMID: 38556522; PMCID: PMC10982290. doi: 10.1038/s41598-024-58436-6.

Zouaoui, K. B., Cauchie, P., Remacle, C., Guillaume, M., Brohée, D., Hubert, J., & Vanhaeverbeek, M. (2002). A new device for measurement of fibrin clot lysis: Application to the Euglobulin Clot Lysis Time. BMC Biotechnology2(1), 8.