Factor VII

Assay overview

Nodia has a broad range of Factor VII, anti-Factor VII and activated Factor VII (FVIIa) assays for both clinical and pharmaceutical applications. Find out more about our tests on this Factor VII specific page.

The key coagulation factor FVII, and its activated form FVIIa, present a major interest for their role at the initiation phase of blood coagulation, and because they can activate all blood coagulation cascade, through the extrinsic, but also the intrinsic pathway.

J. Amiral et al. – 2017

Factor VII

Introduction

The HEMOCLOT™ FVII reagent method is a clotting assay where all the extrinsic pathway clotting factors are present and in excess, except for FVII, which is brought by the diluted tested plasma.
FVII is the limiting factor and clotting time obtained is inversely
proportional to the concentration of FVII.

Intended Use

The HEMOCLOT™ FVII reagent is proposed for measuring FVII in any clinical situation where it can be deficient:

    • Vitamin K deficiency (hepatic diseases, primary biliary cirrhosis, deficiency in new-borns, antibiotherapy, …).
    • Vitamin K antagonists (dicoumarol therapy, …).
    • Isolated deficiencies of FVII.
    • Accelerated clotting factor consumption (DIC).
    •  
Reaction principle

Major Features

As contrary to factor deficient plasma, the Hemoclot FVII reagent is not depleted from a single factor, but is build  with all the extrinsic factors needed to obtain a clot, except for FVII. Deficiencies in the plasma sample other then Factor VII will not influence the end result in any way.

CE marked applications for normal and low range are available for all common coagulation analyzers.

Introduction

The BIOPHEN™ FVII kit is a chromogenic method for quantitative determination of the Factor VII (FVII) activity in purified medium or citrated plasma using a chromogenic method, manual or automated.

Intended Use

The BIOPHEN™ FVII kit is intend for pharmaceutical testing of purified medium or citrated plasma for Factor VII.

Reaction principle

Major Features

The BIOPHEN FVII kit is a stable chromogenic assay, easy to automate with a broad dynamic range to fit all your applications.

Factor VII antigen

Activated Factor VII

Introduction

Hyphen Biomed has developed two different assays for Factor VIIa : the Hemoclot Factor VIIa clotting assay and the chromogenic BIOPHEN Factor VIIa.  Each assay has specific applications such as the detection of FVIIa in therapeutics,  the follow-up of treated patients, including haemophiliacs with inhibitors, patients with severe bleeding risk etc.

For more information regarding this topic, please read the Factor VIIa article published in 2017 by dr. J. Amiral et all. (DOI: 10.1016/j.transci.2016.12.029).

Introduction

The HEMOCLOT™ Factor VIIa kit is a clotting assay for the quantitative determination of activated Factor VII (FVIIa) activity, in purified medium or citrated plasma, using manual or automated method. It is insensitive to Factor VII.

Reaction principle
Intended Use

Measuring Factor VIIa activity in plasma samples in normal and pathophysiological conditions.

Major Features

  • Can be run on the most common laboratory instruments. 
  • Standardized thanks to the use of the FVIIa concentrate WHO International Standard (NIBSC).

Introduction

The BIOPHEN™ Factor VIIa kit is a chromogenic method intended for in vitro quantitative determination of activated Factor VII (FVIIa) activity in purified milieu, using manual or automated method.

Reaction principle
Intended Use

This kit is designed for FVIIa potency assigning in purified systems.

Major Features

  • Can be run on the most common laboratory instruments. 
  • Standardized thanks to the use of the FVIIa concentrate WHO International Standard (NIBSC).

Controls and Calibrators

A set of specific Factor VIIa calibrators and controls is available and can be purchased separately.

Factor VII Inhibitor and Deficient plasma

Factor VII deficient plasma products

The Factor VII Deficient Plasma can be used for the quantitative determination of Factor VII activity in human citrated plasma using a clotting assay via a manual or automated method.

In case Frozen Deficient plasma is preferred, you can chose for 5-Diagnostics Human Factor VII Deficient Plasma, Frozen.

Factor VII inhibitor plasma products

All Factor VII Inhibitor Plasmas are produced from normal human plasmas from which specific factors have been removed by selective affinity immuno-adsorption and an antibody inhibitory to the specific factor is added to provide neutralizing activity.

Product overview

ReferenceProduct namePackagingCategoriesManufacturer
221304BIOPHEN™ Factor VII2 x 80 testsChromogenic AssayHYPHEN Biomed
221312BIOPHEN™ Factor VIIa2 x 4mlChromogenic AssayHYPHEN Biomed
CK051KHEMOCLOT™ Factor VII and Factor X – small6 x 1mlClotting AssayHYPHEN Biomed
CK051LHEMOCLOT™ Factor VII and Factor X – large20 x 1mlClotting AssayHYPHEN Biomed
CK081KHEMOCLOT™ Factor VII Reagent6 x 1mlClotting AssayHYPHEN Biomed
CK092KHEMOCLOT™ Factor VIIa3 x 2mlClotting AssayHYPHEN Biomed
RK036AZYMUTEST™ Factor VII96 testsELISAHYPHEN Biomed
5D-551185-D ELISA Factor VII:Ag ELISA Kit96 testsELISA5-Diagnostics
223201BIOPHEN™ Normal Control Plasma12 x 1mlControlHYPHEN Biomed
223301BIOPHEN™ Abnormal Control Plasma12 x 1mlControlHYPHEN Biomed
224601BIOPHEN™ FIXa Control set2x (6 x 1ml)ControlHYPHEN Biomed
226301BIOPHEN™ Calibrator Factor VIIa6 x 2mlCalibratorHYPHEN Biomed
224901BIOPHEN™ Factor VIIa Control set6 x 1mlControlHYPHEN Biomed
DP030AFactor VII Deficient Plasma – small1mlDeficient PlasmaHYPHEN Biomed
DP030KFactor VII Deficient Plasma – large6 x 1mlDeficient PlasmaHYPHEN Biomed
5D-47118FHuman Factor VII Inhibitor Plasma, Mild, Frozen1mLInhibitor Plasma5-Diagnostics
5D-48118FHuman Factor VII Inhibitor Plasma, Moderate, Frozen1mLInhibitor Plasma5-Diagnostics
5D-49118FHuman Factor VII Inhibitor Plasma, Severe, Frozen1mLInhibitor Plasma5-Diagnostics
PP013AHuman Factor VII100ug ProteinHYPHEN Biomed
5D-10118SAnti-human Factor VII, Sheep, Purified IgG10mgAntibodies5-Diagnostics
5D-11118SAnti-human Factor VII, Sheep, Affinity Purified IgG0.5mgAntibodies5-Diagnostics
5D-12118SAnti-human Factor VII, Sheep, HRP conjugated IgG0.2mgAntibodies5-Diagnostics
5D-16118SAnti-human Factor VII, Sheep, Affinity Purified FITC Conjugated IgG0.1mgAntibodies5-Diagnostics
5D-17118SAnti-human Factor VII, Sheep, Affinity Purified Biotinylated IgG0.1mgAntibodies5-Diagnostics
5D-181185-EIA Anti-human Factor VII, Matched Pair Antibodies5 platesAntibodies5-Diagnostics