Assay overview
Nodia has a broad range of Factor VII, anti-Factor VII and activated Factor VII (FVIIa) assays for both clinical and pharmaceutical applications. Find out more about our tests on this Factor VII specific page.
“The key coagulation factor FVII, and its activated form FVIIa, present a major interest for their role at the initiation phase of blood coagulation, and because they can activate all blood coagulation cascade, through the extrinsic, but also the intrinsic pathway.“
J. Amiral et al. – 2017
Factor VII
Introduction
The HEMOCLOT™ FVII reagent method is a clotting assay where all the extrinsic pathway clotting factors are present and in excess, except for FVII, which is brought by the diluted tested plasma.
FVII is the limiting factor and clotting time obtained is inversely
proportional to the concentration of FVII.
Intended Use
The HEMOCLOT™ FVII reagent is proposed for measuring FVII in any clinical situation where it can be deficient:
- Vitamin K deficiency (hepatic diseases, primary biliary cirrhosis, deficiency in new-borns, antibiotherapy, …).
- Vitamin K antagonists (dicoumarol therapy, …).
- Isolated deficiencies of FVII.
- Accelerated clotting factor consumption (DIC).
Reaction principle
Major Features
As contrary to factor deficient plasma, the Hemoclot FVII reagent is not depleted from a single factor, but is build with all the extrinsic factors needed to obtain a clot, except for FVII. Deficiencies in the plasma sample other then Factor VII will not influence the end result in any way.
CE marked applications for normal and low range are available for all common coagulation analyzers.
Introduction
The BIOPHEN™ FVII kit is a chromogenic method for quantitative determination of the Factor VII (FVII) activity in purified medium or citrated plasma using a chromogenic method, manual or automated.
Intended Use
The BIOPHEN™ FVII kit is intend for pharmaceutical testing of purified medium or citrated plasma for Factor VII.
Reaction principle
Major Features
The BIOPHEN FVII kit is a stable chromogenic assay, easy to automate with a broad dynamic range to fit all your applications.
Factor VII antigen
Sandwich ELISA assay for measuring human Factor VII (FVII) antigen in plasma, or in any fluid where FVII can be present. Assay designed with a rabbit polyclonal antibody specific for FVII for coating the ELISA plate and preparation of horse radish peroxidase (HRP).
DIY
Antibodies for immunoblotting, immunostaining of cells and several types of immunoassays where a higher signal-to-noise is required:
- Anti-human Factor VII, Sheep, Affinity Purified Biotinylated IgG
- Anti-human Factor VII, Sheep, Affinity Purified FITC Conjugated IgG
- Anti-human Factor VII, Sheep, HRP Conjugated IgG
- Anti-human Factor VII, Sheep, Affinity Purified IgG
- Anti-human Factor VII, Sheep, Purified IgG
Matched pair of antibodies:
Activated Factor VII
Introduction
Hyphen Biomed has developed two different assays for Factor VIIa : the Hemoclot Factor VIIa clotting assay and the chromogenic BIOPHEN Factor VIIa. Each assay has specific applications such as the detection of FVIIa in therapeutics, the follow-up of treated patients, including haemophiliacs with inhibitors, patients with severe bleeding risk etc.
For more information regarding this topic, please read the Factor VIIa article published in 2017 by dr. J. Amiral et all. (DOI: 10.1016/j.transci.2016.12.029).
Introduction
The HEMOCLOT™ Factor VIIa kit is a clotting assay for the quantitative determination of activated Factor VII (FVIIa) activity, in purified medium or citrated plasma, using manual or automated method. It is insensitive to Factor VII.
Reaction principle
Intended Use
Measuring Factor VIIa activity in plasma samples in normal and pathophysiological conditions.
Major Features
- Can be run on the most common laboratory instruments.
- Standardized thanks to the use of the FVIIa concentrate WHO International Standard (NIBSC).
Introduction
The BIOPHEN™ Factor VIIa kit is a chromogenic method intended for in vitro quantitative determination of activated Factor VII (FVIIa) activity in purified milieu, using manual or automated method.
Reaction principle
Intended Use
This kit is designed for FVIIa potency assigning in purified systems.
Major Features
- Can be run on the most common laboratory instruments.
- Standardized thanks to the use of the FVIIa concentrate WHO International Standard (NIBSC).
Controls and Calibrators
A set of specific Factor VIIa calibrators and controls is available and can be purchased separately.
Factor VII Inhibitor and Deficient plasma
Factor VII deficient plasma products
The Factor VII Deficient Plasma can be used for the quantitative determination of Factor VII activity in human citrated plasma using a clotting assay via a manual or automated method.
In case Frozen Deficient plasma is preferred, you can chose for 5-Diagnostics Human Factor VII Deficient Plasma, Frozen.
Factor VII inhibitor plasma products
All Factor VII Inhibitor Plasmas are produced from normal human plasmas from which specific factors have been removed by selective affinity immuno-adsorption and an antibody inhibitory to the specific factor is added to provide neutralizing activity.
Product overview
| Reference | Product name | Packaging | Categories | Manufacturer |
|---|---|---|---|---|
| 221304 | BIOPHEN™ Factor VII | 2 x 80 tests | Chromogenic Assay | HYPHEN Biomed |
| 221312 | BIOPHEN™ Factor VIIa | 2 x 4ml | Chromogenic Assay | HYPHEN Biomed |
| CK051K | HEMOCLOT™ Factor VII and Factor X – small | 6 x 1ml | Clotting Assay | HYPHEN Biomed |
| CK051L | HEMOCLOT™ Factor VII and Factor X – large | 20 x 1ml | Clotting Assay | HYPHEN Biomed |
| CK081K | HEMOCLOT™ Factor VII Reagent | 6 x 1ml | Clotting Assay | HYPHEN Biomed |
| CK092K | HEMOCLOT™ Factor VIIa | 3 x 2ml | Clotting Assay | HYPHEN Biomed |
| RK036A | ZYMUTEST™ Factor VII | 96 tests | ELISA | HYPHEN Biomed |
| 5D-55118 | 5-D ELISA Factor VII:Ag ELISA Kit | 96 tests | ELISA | 5-Diagnostics |
| 223201 | BIOPHEN™ Normal Control Plasma | 12 x 1ml | Control | HYPHEN Biomed |
| 223301 | BIOPHEN™ Abnormal Control Plasma | 12 x 1ml | Control | HYPHEN Biomed |
| 224601 | BIOPHEN™ FIXa Control set | 2x (6 x 1ml) | Control | HYPHEN Biomed |
| 226301 | BIOPHEN™ Calibrator Factor VIIa | 6 x 2ml | Calibrator | HYPHEN Biomed |
| 224901 | BIOPHEN™ Factor VIIa Control set | 6 x 1ml | Control | HYPHEN Biomed |
| DP030A | Factor VII Deficient Plasma – small | 1ml | Deficient Plasma | HYPHEN Biomed |
| DP030K | Factor VII Deficient Plasma – large | 6 x 1ml | Deficient Plasma | HYPHEN Biomed |
| 5D-47118F | Human Factor VII Inhibitor Plasma, Mild, Frozen | 1mL | Inhibitor Plasma | 5-Diagnostics |
| 5D-48118F | Human Factor VII Inhibitor Plasma, Moderate, Frozen | 1mL | Inhibitor Plasma | 5-Diagnostics |
| 5D-49118F | Human Factor VII Inhibitor Plasma, Severe, Frozen | 1mL | Inhibitor Plasma | 5-Diagnostics |
| PP013A | Human Factor VII | 100ug | Protein | HYPHEN Biomed |
| 5D-10118S | Anti-human Factor VII, Sheep, Purified IgG | 10mg | Antibodies | 5-Diagnostics |
| 5D-11118S | Anti-human Factor VII, Sheep, Affinity Purified IgG | 0.5mg | Antibodies | 5-Diagnostics |
| 5D-12118S | Anti-human Factor VII, Sheep, HRP conjugated IgG | 0.2mg | Antibodies | 5-Diagnostics |
| 5D-16118S | Anti-human Factor VII, Sheep, Affinity Purified FITC Conjugated IgG | 0.1mg | Antibodies | 5-Diagnostics |
| 5D-17118S | Anti-human Factor VII, Sheep, Affinity Purified Biotinylated IgG | 0.1mg | Antibodies | 5-Diagnostics |
| 5D-18118 | 5-EIA Anti-human Factor VII, Matched Pair Antibodies | 5 plates | Antibodies | 5-Diagnostics |