Product Description
The Glucatell® assay kit is specific for (1→3)-β-D-glucan. The assay is based upon a modification of the Limulus Amebocyte Lysate (LAL) pathway. The Glucatell® reagent is processed to eliminate Factor C, and is therefore specific for (1→3)-β-D-glucan. The reagent does not react to other polysaccharides, including beta-glucans with different glycosidic linkages.
Sample to Lysate Ratio
Kinetic Assay: 25 µL of sample or standard is mixed with 100 µL of reagent in a microplate.
Test Performance
Kinetic Assay: The Glucatell®/sample mixture is incubated at 37°C ± 1°C in a microplate reader equipped with a 405 nm filter and analyzed over time by a suitable software program.
Product Stability
Store all reagents at 2–8°C in the dark. Reconstituted Glucatell® reagent should be stored at 2–8°C and used within 2 hours. Alternatively, reconstituted Glucatell® reagent can be frozen at -20°C for 20 days, thawed once and used.
Product Packaging
The Glucatell® kit is available as either an end-point or kinetic chromogenic assay for use in microplates. The kit contains the Glucatell® reagent, a (1→3)-β-D-glucan standard, buffer, glucan-free water, and glucan-free microplates.
Product Applications
- Analyzing final products for (1→3)-β-D-glucan
- Investigating LAL Out of Specification results
- Qualifying raw materials
- Monitoring cellulosic filter extractables
- Monitoring fungal fermentation processes
- Analyzing fermentation and cell culture media
- Monitoring airborne glucan burden