Pyrotell®-T lysate is used to quantify endotoxin in kinetic turbidimetric tests. Pyrotell®-T lysate can be used with the Pyros Kinetix® Flex tube reader and also incubating microplate readers. When used with the Pyros Kinetix® Flex tube reader, Pyrotell®-T gives a highly economic, flexible and sensitive LAL assay.
It can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.
Chose your instrument:
Plate reader assay
In a microplate reader, the reagent is used at a ratio of 1:1 and a volume of 50 µL/well (100 tests/vial) or 100 µL/well (50 tests/vial).
| Reference | Product name | Packaging | Categories |
|---|---|---|---|
| T0051-5 | Pyrotell®-T (approx. 250 tests) | 5 x 5 ml | Reagens (Lysate) |
| E0005-1 | Escherichia coli 0113:H10 0.5 µg / vial | 1 vial | Control Standard Endotoxin |
| WP0501 | LAL Reagent Water 50 ml | 1 bottle | LRW Water |
| BC051-5 | Pyrosol Buffer without pH indicator – 5.5 ml | 5 x 5.5 ml | Buffer |
| TB240 | Pyrotube D | 40 tubes/pack | Dilution Tubes |
| CA961-10 | Pyroplate 96 well microplate – 10 | 10 plates | Microtiterplate |
| PPT25-10 | Pyroclear Pipette Tips – 250 µl | 10 x 96 tips/box | Pipette tips |
| PPT10-08 | Pyroclear Pipette Tips – 1000 µl | 8 x 96 tips/box | Pipette tips |
| EPP-5 | Combitips – 10 | 10 tips/pack | Repeater pipet tips |
Pyrotell-T lysate is reconstituted with an optimized Pyrosol reconstitution buffer (BC051-5). Pyrotell-T can also be reconstituted with Glucashield buffer, a (1→3)-ß-D-Glucan inhibiting buffer, to render the assay endotoxin specific.
Tube reader assay
In the Pyros Kinetix Flex tube reader, Pyrotell-T can be used at an economical ratio of 4:1 using 50 µL of reagent per well or at 1:1 using 100 µL/well.
| Reference | Product name | Packaging | Categories |
|---|---|---|---|
| T0051-5 | Pyrotell®-T (approx. 250 tests) | 5 x 5 ml | Lysate |
| E0005-1 | Escherichia coli 0113:H10 0.5 µg/vial | 1 vial | CSE |
| WP0501 | LAL Reagent Water 50 ml | 1 bottle | LRW Water |
| BC051-5 | Pyrosol Buffer without pH indicator – 5.5 ml | 5 x 5.5 ml | Buffer |
| TB240 | Pyrotube D | 40 tubes / pack | Dilution Tubes |
| TK050 | Pyrotube K | 50 tubes / pack | PKFlex Test Tubes |
| PPT25-10 | Pyroclear Pipette Tips – 250 µl | 10 x 96 tips / box | Pipette tips |
| PPT10-08 | Pyroclear Pipette Tips – 1000 µl | 8 x 96 tips / box | Pipette tips |
| EPP-5 | Combitips – 10 | 10 / pack | Repeater pipet tips |
Pyrotell-T lysate is reconstituted with an optimized Pyrosol reconstitution buffer (BC051-5). Pyrotell-T can also be reconstituted with Glucashield buffer, a (1→3)-ß-D-Glucan inhibiting buffer, to render the assay endotoxin specific.
