Turbidimetric Method

Pyrotell®-T lysate is used to quantify endotoxin in kinetic turbidimetric tests. Pyrotell®-T lysate can be used with the Pyros Kinetix® Flex tube reader and also incubating microplate readers. When used with the Pyros Kinetix® Flex tube reader, Pyrotell®-T gives a highly economic, flexible and sensitive LAL assay.

It can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.

Chose your instrument:

Plate reader assay

In a microplate reader, the reagent is used at a ratio of 1:1 and a volume of 50 µL/well (100 tests/vial) or 100 µL/well (50 tests/vial). 

ReferenceProduct namePackagingCategories
T0051-5Pyrotell®-T (approx. 250 tests)5 x 5 mlReagens (Lysate)
E0005-1Escherichia coli 0113:H10 0.5 µg / vial1 vialControl Standard Endotoxin
WP0501LAL Reagent Water 50 ml1 bottleLRW Water
BC051-5Pyrosol Buffer without pH indicator – 5.5 ml5 x 5.5 mlBuffer
TB240Pyrotube D40 tubes/packDilution Tubes
CA961-10Pyroplate 96 well microplate – 1010 platesMicrotiterplate
PPT25-10Pyroclear Pipette Tips – 250 µl10 x 96 tips/boxPipette tips
PPT10-08Pyroclear Pipette Tips – 1000 µl8 x 96 tips/boxPipette tips
EPP-5Combitips – 1010 tips/packRepeater pipet tips

Pyrotell-T lysate is reconstituted with an optimized Pyrosol reconstitution buffer (BC051-5). Pyrotell-T can also be reconstituted with Glucashield buffer, a (1→3)-ß-D-Glucan inhibiting buffer, to render the assay endotoxin specific.

Tube reader assay

In the Pyros Kinetix Flex tube reader, Pyrotell-T can be used at an economical ratio of 4:1 using 50 µL of reagent per well or at 1:1 using 100 µL/well.

ReferenceProduct namePackagingCategories
T0051-5Pyrotell®-T (approx. 250 tests)5 x 5 mlLysate
E0005-1Escherichia coli 0113:H10 0.5 µg/vial1 vialCSE
WP0501LAL Reagent Water 50 ml1 bottleLRW Water
BC051-5Pyrosol Buffer without pH indicator – 5.5 ml5 x 5.5 mlBuffer
TB240Pyrotube D40 tubes / packDilution Tubes
TK050Pyrotube K50 tubes / packPKFlex Test Tubes
PPT25-10Pyroclear Pipette Tips – 250 µl10 x 96 tips / boxPipette tips
PPT10-08Pyroclear Pipette Tips – 1000 µl8 x 96 tips / boxPipette tips
EPP-5Combitips – 1010 / packRepeater pipet tips

Pyrotell-T lysate is reconstituted with an optimized Pyrosol reconstitution buffer (BC051-5). Pyrotell-T can also be reconstituted with Glucashield buffer, a (1→3)-ß-D-Glucan inhibiting buffer, to render the assay endotoxin specific.