Chromogenic Method

Pyrochrome is a versatile quantitative reagent that is used to perform kinetic or endpoint assays. It is a sensitive and flexible reagent that can be used for testing in compliance with the USP, EP and JP bacterial endotoxins test chapter. Pyrochrome is offered with either Pyrochrome Buffer or for endotoxin-specific testing, with Glucashield® Buffer. Pyrochrome can be used with the new Pyros Kinetix® Flex tube reader at a 1:1 and economical 4:1 ratio.

Pyrochrome can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.

Chose your instrument:

Plate reader assay

In a microplate reader, the reagent is used at a ratio of 1:1 and a volume of 50 µL/well (60 tests/vial) or 100 µL/well (30 tests/vial). 
ReferenceProduct namePackagingCategories
C1500-5Pyrochrome® with Reconstitution Buffer
(approx.160 tests)
5 x 3.2 mlReagent (lysate)
EC010-5Escherichia coli 0113:H10 10 ng / vial5 vialsControl Standard Endotoxin
WP0501LAL Reagent Water 50 ml1 bottleLRW Water
TB240Pyrotube D40 tubes / packDilution Tubes
CA961-10Pyroplate 96 well microplate – 1010 platesMicrotiterplate
PPT25-10Pyroclear Pipette Tips – 250 µl 10 x 96 tips/boxPipette tips
PPT10-08Pyroclear Pipette Tips – 1000 µl8 x 96 tips / boxPipette tips
EPP-5Combitips – 1010 tips/packRepeater pipet tips
 
Pyrochrome lysate is reconstituted with an optimized Pyrochome reconstitution buffer (C1500). Pyrochrome can also be reconstituted with Glucashield buffer, a (1→3)-ß-D-Glucan inhibiting buffer, to render the assay endotoxin specific (CG1500).

Tube reader assay

In the Pyros Kinetix Flex tube reader, Pyrochrome can be used at an economical ratio of 4:1 using 50 µL of reagent per well or at 1:1 using 100 µL/well.
ReferenceProduct namePackagingCategories
C1500-5Pyrochrome® with Reconstitution Buffer
(approx.160 tests)
5 x 3.2 mlReagent (Lysate)
EC010-5Escherichia coli 0113:H10 10 ng / vial5 vialsControl Standard Endotoxin
WP0501LAL Reagent Water 50 ml1 bottleLRW Water
BC051-5Pyrosol Buffer without pH indicator – 5.5 ml5 x 5.5 mlBuffer
TB240Pyrotube D40 tubes / packDilution Tubes
TK050Pyrotube K50 tubes / packPKFlex Test Tubes
PPT25-10Pyroclear Pipette Tips – 250 µl10 x 96 tips / boxPipette tips
PPT10-08Pyroclear Pipette Tips – 1000 µl8 x 96 tips / boxPipette tips
EPP-5Combitips – 1010 / packRepeater pipet tips
Pyrochrome lysate is reconstituted with an optimized Pyrochome reconstitution buffer (C1500). Pyrochrome can also be reconstituted with Glucashield buffer, a (1→3)-ß-D-Glucan inhibiting buffer, to render the assay endotoxin specific (CG1500).

Alternative reagents

ChromoLAL

Chromo-LAL lysate is lyophilized with substrate reagent and buffers. It is optimized for the kinetic chromogenic LAL test method in microplate readers. Chromo-LAL is a buffered, stable and robust lysate, suitable for quantitative testing of a wide range of samples.

ReferenceProduct namePackagingCategories
C0031-5Chromo-LAL (approx.150 tests)5×3.2ml
Reagent (Lysate)
E0005-1Escherichia coli 0113:H10 0.5µg/vial1 vialControl Standard Endotoxin

PyroSmart NextGen

PyroSmart NextGen™ recombinant Cascade Reagent (rCR) marks the introduction of a new sustainable recombinant LAL reagent technology for Bacterial Endotoxin Testing (BET). Utilizing the same LAL cascade as traditional LAL reagents. PyroSmart NextGen™ delivers all of the quality and consistency of results you have come to expect from ACC LAL reagents.

PyroSmart NextGen™ can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.

ReferenceProduct namePackagingCategories
PNG050-2PyroSmart NextGen (approx.100 tests)2.8ml/vialReagent (Lysate)
EC010-5Escherichia coli 0113:H10 10 ng / vial5 vialsControl Standard Endotoxin